Quality improvement of UM reconstructions by nonblind deconvolution. (A) Resin-embedded latex beads for measuring the point-spread function (FluoSpheres 1 µm, 488 nm; Invitrogen). Objective: Olympus Fluar 10×, NA 0.3 with 4× postmagnification. (B) PSF determined by averaging the bead recordings shown in A. (C) 3D reconstruction of a part of the right hindlimb of a mouse embryo E12.5. (D) Deconvolution of C using a measured PSF. In contrast to C even fine nerve branches are distinguishable. (C,D, Reprinted, with permission, from Jährling et al. 2008, ©Elsevier GmbH.)