Nuclear Patch-Clamp Electrophysiology of Ca2+ Channels
- 1Department of Physiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104;
- 2Department of Physiology, University of Hong Kong, Hong Kong
Abstract
Patch-clamping the outer or inner nuclear membrane of isolated nuclei is very similar to patch-clamping the plasma membrane of isolated cells. This protocol describes in detail all the steps required to successfully obtain nuclear membrane patches, in various configurations, from both the outer and inner nuclear membranes of isolated nuclei.
Footnotes
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↵3 Correspondence: foskett{at}mail.med.upenn.edu
- © 2013 Cold Spring Harbor Laboratory Press
