Labeling Individual Neurons in the Brains of Live Xenopus Tadpoles by Electroporation of Dyes or DNA
Adapted from Imaging in Developmental Biology (ed. Sharpe and Wong). CSHL Press, Cold Spring Harbor, NY, USA, 2011.Abstract
This protocol describes the targeted introduction of fluorophore in the form of a dye or genetic material into single cells. This method has the advantage of producing true single-cell chimeric animals in which to study the effects of overexpression or knockdown of a gene in an otherwise entirely wild-type background.
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