Protocol

Simultaneous Visualization of Multiple Protein Interactions Using Multicolor Bimolecular Fluorescence Complementation (BiFC) Analysis

  1. Tom K. Kerppola
Adapted from Imaging: A Laboratory Manual (ed. Yuste). CSHL Press, Cold Spring Harbor, NY, USA, 2011.

Abstract

This multicolor bimolecular fluorescence complementation procedure is designed for the analysis of alternative protein interactions in cultured mammalian cells, but it can be readily adapted to any cell type or aerobically grown organism that can be genetically modified to express the fusion proteins.

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  1. doi:10.1101/pdb.prot077172 Cold Spring Harb Protoc 2013: pdb.prot077172- © 2013 Cold Spring Harbor Laboratory Press
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