A Myeloid-Derived Suppressor Cell–Mediated T-Cell Suppression Assay for Functional Evaluation of Immune Cells in Tumor-Bearing Mice
- 1Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104;
- 2Abramson Cancer Center, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104;
- 3Division of Hematology-Oncology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104
Abstract
This protocol describes a procedure to evaluate the ability of Gr-1+ CD11b+ myeloid-derived suppressor cells (MDSCs) isolated from the spleen or tumor of a tumor-bearing mouse to suppress antigen-specific T-cell proliferation. MDSCs are cultured with responder splenocytes from an OT-1 T-cell receptor transgenic mouse stimulated with cognate peptide (OVA-257 peptide) or with an irrelevant, control peptide. This protocol spans a 4-d period. The readout is T-cell proliferation by 3[H]-thymidine incorporation during an 18-h pulse between Days 3 and 4.
Footnotes
-
↵4 Correspondence: rhv{at}exchange.upenn.edu
- © 2013 Cold Spring Harbor Laboratory Press
