High-Ovomucoid Stock Solution (6×)
BSA (Sigma-Aldrich A8806)
D-PBS (Thermo Scientific HyClone SH3026401)
NaOH (1 n)
Trypsin inhibitor (Worthington LS003086)
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1. Add 6 g of BSA to 150 mL D-PBS.
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2. Add 6 g of trypsin inhibitor and mix to dissolve.
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3. Add at least 1.5 mL of 1 n NaOH to adjust the pH; continue adding NaOH as necessary to bring up the pH to 7.4.
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4. Bring the volume to 200 mL with D-PBS.
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5. Filter-sterilize through a 0.22-µm filter.
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6. Make 1.0-mL aliquots and store at −20°C.
- © 2014 Cold Spring Harbor Laboratory Press
