Buffer A for Isolation of Mitochondria from Rat Liver
| Reagent | Final concentration |
|---|---|
| EDTA–NaOH (pH 7) | 0.1 mm |
| HEPES–KOH (pH 7.2) | 10 mm |
| Leupeptin | 1 µg/mL |
| Phenylmethanesulfonyl fluoride (PMSF) | 0.15 µm |
| Sucrose | 250 mm |
| Prepare fresh from stock solutions before use. | |
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