LDAO Solution for Purification of VDAC1 from Mitochondria
| Reagent | Final concentration |
|---|---|
| EGTA | 0.2 mm |
| LDAO | 2% |
| Leupeptin | 0.5 µg/mL |
| Phenylmethanesulfonyl fluoride (PMSF) | 0.15 mm |
| Tris–HCl (pH 7.4) | 10 mm |
| Prepare fresh from stock solutions before use. | |
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