Triton X-100 Solution for Purification of VDAC1 from Mitochondria
| Reagent | Final concentration |
|---|---|
| EGTA | 0.2 mm |
| Leupeptin | 0.5 µg/mL |
| Phenylmethanesulfonylfluoride (PMSF) | 0.15 µm |
| Tris–HCl (pH 7.4) | 10 mm |
| Triton X-100 | 3% |
| Prepare fresh from stock solutions before use. | |
- © 2014 Cold Spring Harbor Laboratory Press
