Protocol

Synthesis of NAADP-AM as a Membrane-Permeant NAADP Analog

  1. Grant C. Churchill
  1. Department of Pharmacology, University of Oxford, Oxford OX1 3QT, United Kingdom

    Abstract

    Nicotinic acid adenine dinucleotide phosphate (NAADP), like the other major messengers for Ca2+ mobilization, is passively membrane-impermeant. Instead, a cell-permeant acetoxymethyl ester derivative of NAADP (NAADP-AM) can be synthesized as described here and used to study NAADP-mediated Ca2+ release.

    Footnotes

    • ↵1 Correspondence: antony.galione{at}pharm.ox.ac.uk

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    1. doi:10.1101/pdb.prot076927 Cold Spring Harb Protoc 2014: pdb.prot076927- © 2014 Cold Spring Harbor Laboratory Press
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