Myelination Medium (MyM)

• 1. Dilute a 10 µg/mL stock solution of CNTF to 0.1 ng/mL by combining the following:

  • 1 µL CNTF stock (10 µg/mL)

  • 99 µL MyM base medium

• 2. Add 15 µL of 0.1 ng/mL CNTF to the following:

  • 15 mL MyM base medium

  • 15 µL BDNF (Peprotech 450-02; 50 µg/mL in D-PBS [Gibco 14287-080] containing 0.2% BSA)

• 3. Equilibrate in an incubator to 37°C/10% CO₂.

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