Recipe

MS Homogenization Buffer (1×)

210 mm mannitol

70 mm sucrose

5 mm Tris-HCl (pH 7.5)

1 mm EDTA (pH 7.5)

The buffer should be ice cold before use.

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  1. doi:10.1101/pdb.rec080556 Cold Spring Harb Protoc 2014: pdb.rec080556- © 2014 Cold Spring Harbor Laboratory Press
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