Recipe

MS Homogenization Buffer (2.5×)

525 mm mannitol

175 mm sucrose

12.5 mm Tris-HCl (pH 7.5)

2.5 mm EDTA (pH 7.5)

The buffer should be ice cold before use.

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  1. doi:10.1101/pdb.rec080564 Cold Spring Harb Protoc 2014: pdb.rec080564- © 2014 Cold Spring Harbor Laboratory Press
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