Multi-Channel Imaging of Cellular Signaling: Interplay of Ca2+ and Conventional Protein Kinase C
- 1Institute for Molecular Cell Biology and Research Center for Molecular Imaging and Screening, School of Medicine, Saarland University, 66421 Homburg/Saar, Germany;
- 2Cell Biology and Biophysics Unit, EMBL, 69117 Heidelberg, Germany
Abstract
To investigate the coupling between the calcium (Ca2+) signal and the conventional protein kinase C alpha (PKCα) translocation, which are both transient processes, a sectioning imaging technique with sufficient scanning speed is required. Here, we describe how to use a Nipkow-disk-based confocal system with an image splitter for two cameras to acquire simultaneously both the Ca2+ signal and images of PKCα fused to the fluorescent protein DsRed2 in HEK293 cells on ATP stimulation.
Footnotes
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↵3 Correspondence: lars_kaestner{at}me.com
- © 2014 Cold Spring Harbor Laboratory Press
