Recipe

Buffer A-FS

  • 100 mm 2-(N-morpholino)ethanesulfonic acid (MES) (pH 6.5, adjusted with NaOH)

  • 0.2 mm EGTA

  • 0.5 mm MgCl2

  • 12.5% (w/v) Ficoll PM400 (GE Healthcare)

  • 12.5% (w/v) sucrose

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  1. doi:10.1101/pdb.rec084723 Cold Spring Harb Protoc 2014: pdb.rec084723- © 2014 Cold Spring Harbor Laboratory Press
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