First-Strand Buffer for RT–PCR (5×)
| Reagent | Quantity (for 1 mL) | Final concentration |
|---|---|---|
| Tris–HCl (1 m, pH 8.3) | 250 µL | 0.25 m |
| KCl (1 m) | 375 µL | 375 mm |
| MgCl2 (1 m) | 15 µL | 15 mm |
| DTT (0.5 m) | 200 µL | 0.1 m |
| H2O | 160 µL | |
| Store for up to 6–12 mo at –20°C. | ||
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