Artificial Cerebrospinal Fluid (aCSF) for Zebra Finch Brain
| Reagent | Final concentration |
|---|---|
| NaCl | 199 mm |
| NaHCO3 | 26.2 mm |
| KCl | 2.5 mm |
| MgSO4 | 1.0 mm |
| CaCl2 | 2.5 mm |
| Glucose | 11.0 mm |
| Dissolve the reagents in ultrapure ddH2O (∼18 MΩ) and adjust the pH to 7.4–7.6. Store at 4°C in the dark for a maximum of 4 wk. Add 1% bovine serum albumin (BSA; Sigma-Aldrich A1470) to 50-mL aliquots and filter (with 0.2-μm syringe filters) to eliminate suspended particles and reduce clogging of FEP tubing and probes. | |
- © 2014 Cold Spring Harbor Laboratory Press
