Column Washing Buffer
| Reagent | Amount for 50 mL | Final Concentration |
|---|---|---|
| Tris–HCl (1 m, pH 7.5) | 0.5 mL | 10 mm |
| NaCl (5 m) | 0.5 mL | 50 mm |
| EDTA (0.5 m, pH 8.0) | 0.01 mL | 0.10 mm |
| DEPC-treated H2O | to 50 mL | - |
| Prepare all solutions with certified nuclease-free H2O or DEPC-treated H2O. Store at room temperature. | ||
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