SSPE (20×, pH 7.4)
| Reagent | Amount for 1 L | Final Concentration |
|---|---|---|
| NaCl | 175.3 g | 3 m |
| NaH2PO4 · H2O | 27.6 g | 0.2 m |
| EDTA | 7.4 g | 0.02 m |
| DEPC-treated H2O | to 1 L | |
| Adjust the pH to 7.4 with 10 n NaOH. Store at room temperature. | ||
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