Transcription Buffer (10×)
| Reagent | Amount for 10 mL | Final Concentration |
|---|---|---|
| Tris–HCl (1 m, pH 7.5) | 4 mL | 400 mm |
| MgCl2 (1 m) | 0.6 mL | 60 mm |
| Spermidine (1 m) | 0.2 mL | 20 mm |
| DEPC-treated H2O | to 10 mL | |
| Use solutions prepared with certified nuclease-free H2O or DEPC-treated H2O. Store in 10-µL aliquots at −20°C. | ||
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