Recipe

TNT Solution

Reagent Amount for 500 mL Final Concentration
Tris–HCl (1 m, pH 7.5)   50 mL  100 mm
NaCl (5 m)   15 mL  150 mm
Triton X-100   1.5 mL 0.3% (v/v)
H2O to 500 mL –
Store at room temperature.
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  1. doi:10.1101/pdb.rec085415 Cold Spring Harb Protoc 2014: pdb.rec085415- © 2014 Cold Spring Harbor Laboratory Press
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