Recipe

Wash Buffer I

Reagent Amount for 600 mL Final Concentration
SSPE (20×, prepared in DEPC-treated H2O)    60 mL    2×
Formamide   300 mL 50% (v/v)
DEPC-treated H2O   to 600 mL     –

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  1. doi:10.1101/pdb.rec085423 Cold Spring Harb Protoc 2014: pdb.rec085423- © 2014 Cold Spring Harbor Laboratory Press
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