Topic Introduction

Purification of Schwann Cells

  1. Amanda Brosius Lutz1
  1. Stanford University School of Medicine, Department of Neurobiology, Stanford, California 94305

    Abstract

    This article introduces methods for the acute purification and primary culture of Schwann cells from the mouse sciatic nerve. Immunopanning can be used to isolate Schwann cells from intact nerves during early postnatal development as well as to purify Schwann cells from adult nerves following sciatic nerve injury. These methods facilitate the exploration of mouse Schwann cell biology in the healthy and injured peripheral nerve.

    Footnotes

    • ↵1 Correspondence: abrolutz{at}stanford.edu

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    1. doi:10.1101/pdb.top073981 Cold Spring Harb Protoc 2014: pdb.top073981- © 2014 Cold Spring Harbor Laboratory Press
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