Recipe

Permeabilization Solution for Measuring Ca2+ Fluxes across the Mitochondrial Inner Membrane

Reagent Final concentration
Cyclosporin A   1 µm
Digitonin  0.007%
EGTA  0.4 mm
HEPES–KOH (pH 7.4)   10 mm
KH2PO4  2.5 mm
Succinate   5 mm
Sucrose  220 mm
Prepare fresh from stock solutions before use.
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  1. doi:10.1101/pdb.rec076224 Cold Spring Harb Protoc 2014: pdb.rec076224- © 2014 Cold Spring Harbor Laboratory Press
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