Producing and Concentrating Lenti-Cre for Mouse Infections
- 1Molecular Pathology Unit and Center for Cancer Research, Massachusetts General Hospital, Department of Pathology, Harvard Medical School, Charlestown, Massachusetts 02129;
- 2Koch Institute for Integrative Cancer Research and Department of Biology, Massachusetts Institute of Technology, Howard Hughes Medical Institute, Cambridge, Massachusetts 02139
Abstract
Lentiviral vectors offer versatility as vehicles for gene delivery. They can transduce a wide range of cell types and integrate into the host genome, which results in long-term expression of the transgene (Cre) both in vitro and in vivo. This protocol describes how lentiviral particles are produced, purified, and concentrated.
Footnotes
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↵3 Correspondence: khaigis{at}partners.org
- © 2014 Cold Spring Harbor Laboratory Press
