Avertin (20 mg/mL)

Recipe

1. To prepare a 1.6 g/mL avertin stock solution, add 15.5 mL of 2-methyl-2-butanol (tert-amyl alcohol) to 25 g of avertin (2-2-2 tribromoethanol). Seal the bottle and protect the solution from light. Stir the mixture overnight at room temperature to dissolve.
- Discard the solution if it turns yellow. The stock solution is stable at room temperature for ∼1 yr.
2. To prepare an avertin working solution at 20 mg/mL, dilute the avertin stock solution in phosphate-buffered saline (PBS). Protect the solution from light, and stir overnight at room temperature. Sterilize the working solution by passing through a 0.22-μm filter. Aliquot and store at 4°C in the dark for up to ∼4 mo.