Protease Inhibitor Cocktail (1000×)
| Reagent | Quantity |
|---|---|
| Aprotinin | 5 mg |
| Leupeptin | 5 mg |
| Pepstatin | 5 mg |
| Chymotrypsin | 5 mg |
| Antipain | 5 mg |
| DMSO | 7 mL |
| H2O | 3 mL |
| Combine in a 15-mL screw-cap polypropylene tube and mix overnight at room temperature. Aliquot and store at −20°C. | |
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