Splicing Mix for Mobility Shift Assays (4×)
| Reagent | Quantity (for 100 µL) | Final concentration (4×) |
|---|---|---|
| Creatine phosphate (0.2 m) | 10 µL | 20 mm |
| HEPES/KOH (1 m; pH 7.6) | 8 µL | 80 mm |
| MgCl2 (100 mm) | 12 µL | 12 mm |
| ATP (100 mm) | 8 µL | 8 mm |
| Dithiothreitol (100 mm) | 40 µL | 40 mm |
| H2O | 22 µL | |
| Prepare fresh for each use. Keep on ice. | ||
- © 2014 Cold Spring Harbor Laboratory Press
