Recipe

HEPES-KRH Buffer

Reagent Final concentration
NaCl 116 mm
KCl  4 mm
MgCl2  1 mm
CaCl2  1.8 mm
Glucose  25 mm
HEPES acid  10 mm
Adjust pH to 7.4.
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  1. doi:10.1101/pdb.rec082669 Cold Spring Harb Protoc 2014: pdb.rec082669- © 2014 Cold Spring Harbor Laboratory Press

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