Low-Salt Solution Buffer
| Reagent | Amount (for 50 mL) | Final concentration |
|---|---|---|
| Tris–HCl (1 m, pH 8.0) | 500 µL | 10 mm |
| EDTA (0.5 m, pH 8.0) | 100 µL | 1 mm |
| NaCl (5 m) | 1.5 mL | 150 mm |
| Triton X-100 (10%) | 5 mL | 1% |
| Double-distilled water | 42.9 mL | – |
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