Recipe

PBS/EDTA/EGTA

Reagent Quantity (for 500 mL) Final concentration
Phosphate-buffered saline        (PBS) (10×)   50 mL  1×
EDTA (0.5 m, pH 8.0)   1 mL 1 mm
EGTA (0.1 m, pH 8.0)   5 mL 1 mm
H2O to 500 mL
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  1. doi:10.1101/pdb.rec082826 Cold Spring Harb Protoc 2014: pdb.rec082826- © 2014 Cold Spring Harbor Laboratory Press

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