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The piggyBac system can be used for the genomic integration of transgenes. Combinations of piggyBac donor and helper plasmids direct transgenesis in different cell types, as illustrated here. This month's issue includes a protocol for delivering a binary piggyBac transposon plasmid system into the mammalian brain by in utero electroporation (doi: 10.1101/pdb.prot073650). Image courtesy of Fuyi Chen and Joseph LoTurco.