Protocol

Preparation of Crude Rough Microsomes from Dog Pancreas

  1. David D. Sabatini1
  1. Department of Cell Biology, NYU Medical School, New York, New York 10016

    Abstract

    This protocol describes how to prepare rough microsomes from dog pancreas. These microsomes can be used to analyze mechanisms of protein translocation and membrane insertion.

    Footnotes

    • ↵1 Correspondence: david.sabatini{at}nyumc.org

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    1. doi:10.1101/pdb.prot079988 Cold Spring Harb Protoc 2014: pdb.prot079988- © 2014 Cold Spring Harbor Laboratory Press
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