Recipe

Ca2+ Mix

Reagent Final concentration
Phosphorylation mix (4×)   2.2×
MgCl2 · 6H2O 5.55 mm
CaCl2 1.11 mm
The Ca2+ mix should be freshly prepared, but the separate stock solutions, except for the phosphorylation mix, can be pre-prepared and stored at 4°C for several months.
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  1. doi:10.1101/pdb.rec078386 Cold Spring Harb Protoc 2014: pdb.rec078386- © 2014 Cold Spring Harbor Laboratory Press
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