Lysis Buffer for Microsome Preparation
| Reagent | Final concentration |
|---|---|
| Tris–HCl (pH 7.5) | 10 mm |
| MgCl2 · 6H2O | 0.5 mm |
| This buffer can be pre-prepared and stored at 4°C for several months. Before use, add a commercially available protease inhibitor cocktail according to the manufacturer's instructions. | |
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