Phosphorylation Mix (4×)
| Reagent | Final concentration |
|---|---|
| KCl | 640 mm |
| HEPES (pH 7.0) | 68 mm |
| NaN3 | 20 mm |
| Dithiothreitol (DTT) | 4 mm |
| The 4× phosphorylation mix should be freshly prepared, but the separate stock solutions can be pre-prepared and stored at 4°C for several months. DDT stock solutions must be stored at −20°C. | |
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