Solution 1M
| Reagent | Final concentration |
|---|---|
| Sucrose | 0.5 m |
| Tris–HCl (pH 7.3) | 10 mm |
| CaCl2 | 40 µm |
| Add fresh before use: | |
| Phenylmethanesulfonyl fluoride (PMSF) | 0.23 mm |
| Dithiothreitol (DTT) | 1 mm |
| Solution 1M can be pre-prepared and stored as 10 mL aliquots at −20°C. | |
- © 2014 Cold Spring Harbor Laboratory Press
