Sample Buffer for Phosphorylation Assay
| Reagent | Final concentration |
|---|---|
| Tris–HCl (pH 7.4) | 150 mm |
| Na-EDTA | 8 mm |
| SDS | 3% |
| Sucrose | 20% |
| Bromophenol blue | 0.14 mg/mL |
| Add fresh before use: | |
| Dithiothreitol (DTT) | 10 mm |
| This sample buffer can be pre-prepared and stored as 1 mL aliquots at −20°C. | |
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