Master Mix for Ca2+-Uptake Assay
| Reagent | Final concentration |
|---|---|
| Imidazole–HCl (pH 7.0) | 40 mm |
| KCl | 95 mm |
| NaN3 | 5 mm |
| MgCl2 · 6H2O | 5 mm |
| EGTA (pH 7.0) | 0.5 mm |
| Potassium oxalate | 5 mm |
| Ruthenium Red | 1 µm |
| [45Ca2+] CaCl2 | 1 µCi/mL |
- © 2014 Cold Spring Harbor Laboratory Press
