Biocytin Staining of Glia and Neurons in Brain Slices
Adapted from Imaging in Neuroscience (ed. Helmchen and Konnerth). CSHL Press, Cold Spring Harbor, NY, USA, 2011.Abstract
This protocol describes the use of biocytin to visualize and distinguish the morphology of glia and neurons in rat brain slices. Patch pipettes are used to load biocytin into different cell types. The slices are subsequently fixed, stained, and mounted in preparation for imaging.
- © 2014 Cold Spring Harbor Laboratory Press
