Recipe

Artificial Seawater (ASW) for Live, Intact Sea Urchin Eggs

Reagent Final concentration
CaCl2  11 mm
KCl  10 mm
MgCl2  40 mm
MgSO4  15 mm
NaCl 435 mm
NaHCO3  2.5 mm
Tris base  7 mm
Tris-HCl  13 mm
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  1. doi:10.1101/pdb.rec078980 Cold Spring Harb Protoc 2014: pdb.rec078980- © 2014 Cold Spring Harbor Laboratory Press
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