Recipe

Lysis Buffer for Protein Extraction

Reagent Amount Final concentration
Tris-buffered saline (TBS; 10×, pH 7.5)   5 mL   1×
EDTA (0.5 m)  100 µL 0.05 m
NP-40 (10%)   5 mL   1%
H2O 39.9 mL
Add one EDTA-free complete protease inhibitor cocktail tablet (Roche)
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  1. doi:10.1101/pdb.rec081273 Cold Spring Harb Protoc 2014: pdb.rec081273- © 2014 Cold Spring Harbor Laboratory Press
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