Topic Introduction

Subcellular Fractionation of Rough Microsomes

  1. David D. Sabatini1
  1. Department of Cell Biology, NYU Medical School, New York, New York 10016

    Abstract

    When eukaryotic cells are homogenized, the rough endoplasmic reticula are converted into small vesicles, called rough microsomes. Strategies for the isolation of rough microsomes are introduced here, as are methods for evaluating the purity and intactness of an isolated rough microsomal fraction.

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    • ↵1 Correspondence: david.sabatini{at}nyumc.org

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    1. doi:10.1101/pdb.top074567 Cold Spring Harb Protoc 2014: pdb.top074567- © 2014 Cold Spring Harbor Laboratory Press
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