Protocol

Separation of Tubulin and Microtubule-Associated Proteins by Ion Exchange Chromatography

  1. Roger D. Sloboda1
  1. Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755

    Abstract

    Conventional liquid chromatography on phosphocellulose (PC) can be used to separate tubulin and microtubule-associated proteins (MAPs). Tubulin is a highly acidic protein and thus does not bind to PC. MAPs, however, do bind to PC and can be eluted with a subsequent salt wash of the column.

    Footnotes

    • ↵1 Correspondence: rds{at}dartmouth.edu

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    1. doi:10.1101/pdb.prot081208 Cold Spring Harb Protoc 2015: pdb.prot081208- © 2015 Cold Spring Harbor Laboratory Press
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