Chelex-Treated Ultrapure H₂O

Mix ∼50 g of Chelex 100 resin (sodium form, 50–100 mesh) with 3–4 L of autoclaved ultrapure water (e.g., Milli-Q) in a large plastic container on a stir plate for at least 18 h. Let the resin settle to the bottom of the container for several hours. Use a bottle-top filter to remove all Chelex from the water, and store the filtered water in a plastic container. The resin can be reused several times.

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