Recipe

HEPES-Buffered Hanks Balanced Salt Solution without Ca2+ (Ca2+-Free HHBSS)

Reagent Final concentration
HBSS (10×, without Ca2+, Mg2+, or phenol red;  Life Technologies) 1×
MgCl2 0.49 mm
MgSO4 0.45 mm
HEPES 20 mm
d-glucose 2 g/L

  • Prepare solution in Chelex-treated ultrapure H2O. Adjust pH to 7.2 and sterilize using a bottle-top 0.22-µm filter. Store solution in a metal-free plastic container.

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    1. doi:10.1101/pdb.rec077263 Cold Spring Harb Protoc 2015: pdb.rec077263- © 2015 Cold Spring Harbor Laboratory Press
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