EGTA (0.5 m) for Ca2+ Biosensor Experiments
Add 19.01 g ethylene glycol bis(b-aminoethyl ether) N,N,N′,N′-tetraacetic acid (EGTA; FW 380.35) to 80 mL of Chelex-treated ultrapure H2O. Stir vigorously, heating slightly (solution does not need to boil). Adjust pH to 8.0, first with NaOH pellets, then with 1 m NaOH solution. Note that EGTA will not go into solution until pH is ∼8.0. Adjust final volume to 0.1 L. Filter-sterilize through a 0.22-μm filter. Store solution in a metal-free plastic container.
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