Recipe

HEPES-Buffered Cell Medium

Reagent Final concentration (mm)
NaCl 140
KCl  5.4
MgCl2  1
CaCl2  2
Glucose  16
HEPES  10
All solutions, reagents, and plastic ware used to prepare this medium should be RNase-free and designated for single-cell analysis only. Adjust the pH to 7.4 with HCl and filter through a 0.2-µm filter. Store at room temperature.

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  1. doi:10.1101/pdb.rec079947 Cold Spring Harb Protoc 2015: pdb.rec079947- © 2015 Cold Spring Harbor Laboratory Press
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