HEPES-Buffered Cell Medium
| Reagent | Final concentration (mm) |
|---|---|
| NaCl | 140 |
| KCl | 5.4 |
| MgCl2 | 1 |
| CaCl2 | 2 |
| Glucose | 16 |
| HEPES | 10 |
| All solutions, reagents, and plastic ware used to prepare this medium should be RNase-free and designated for single-cell analysis only. Adjust the pH to 7.4 with HCl and filter through a 0.2-µm filter. Store at room temperature. | |
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