Recipe

Paclitaxel (10 mm)

Prepare a 10 mm paclitaxel stock solution by dissolving the powder (Millipore/Calbiochem 580555 or Sigma-Aldrich T7191) in DMSO. (Paclitaxel is also soluble in acetonitrile or methanol.) Store in small aliquots at −20°C. Paclitaxel in DMSO is stable to repeated freezing and thawing. However, paclitaxel hydrolyzes in aqueous solution; therefore, only prepare enough paclitaxel-containing buffer for a given experiment.

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  1. doi:10.1101/pdb.rec081877 Cold Spring Harb Protoc 2015: pdb.rec081877- © 2015 Cold Spring Harbor Laboratory Press
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